| Autor: |
Thakur, Rakesh Kr., Rajpal, Vijay Rani, Chaudhary, Manju, Sonkar, Anand, Rao, Satyawada Rama, Kaushal, Pankaj, Dubey, Basant Kumar, Goel, Shailendra, Raina, Soom Nath |
| Zdroj: |
Nucleus (0029568X); Dec2023, Vol. 66 Issue 3, p245-260, 16p |
| Abstrakt: |
Tinospora cordifolia (Giloy), with immense therapeutic properties has been used as a powerful rasayana and immunomodulator in alternative systems of medicine. In the present study, two distinct morphotypes (arbitrarily labeled 1 and 2) were identified in the wild Indian giloy populations. Flow cytometric analysis for DNA quantification revealed 1.5-fold moreDNA (2C = 4.32 pg) in morphotype 2 than in morphotype 1 (2C = 2.8 pg). The cytogenetic analysis showed the somatic cells of morphotype 2 to possess triploid (2n = 3x = 39; first report in giloy) compared to diploid (2n = 2x = 26) chromosome number in morphotype 1. While the diploid T. cordifolia and the allied diploid T. sinensis cytotypes exhibited normal meiosis, the triploids showed highly irregular meiosis with average (10.80 II) number of bivalents per cell outnumbering trivalents (3.52 III) and univalents (6.68 I). The chloroplast PCR–RFLP analysis in the diploid and triploid T. cordifolia, and T. sinensis involving 224 chloroplast genes/intergenic spacers and 28 restriction enzyme combinations revealed the contribution of diploid T. cordifolia as the maternal progenitor of the triploid cytotype. The present study unveils a new wild triploid giloy cytotype with significantly higher biomass of great commercial advantage and relevance in plant breeding and biotechnology. The cultivation of this novel, and vigorous triploid giloy cytotype is anticipated to bridge the gap between the demand and supply of superior, uniform giloy raw material for the Ayurveda industry and will also help minimize batch-to-batch variations commonly observed in Ayurvedic drugs. We have also characterized the triploid cytotypes to understand their nature and origin to shed light on evolutionary genetic differentiation mechanisms in giloy, a species with relatively obscure genetics. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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