Polymorphism but not methylation status in the vitamin D receptor gene contributes to oral mucositis in children.

Autor:	Viana Filho, José Maria Chagas, de Souza, Beatriz Fernandes, Coêlho, Marina de Castro, Valença, Ana Maria Gondim, Persuhn, Darlene Camati, de Oliveira, Naila Francis Paulo
Předmět:	STOMATITIS SCIENTIFIC observation CANCER chemotherapy CROSS-sectional method GENETIC polymorphisms CELL receptors FISHER exact test MANN Whitney U Test DNA methylation VITAMIN D METHOTREXATE HEMATOLOGIC malignancies GENOTYPES DESCRIPTIVE statistics CHI-squared test RESEARCH funding POLYMERASE chain reaction EPIGENOMICS EVALUATION DISEASE complications CHILDREN
Zdroj:	Oral Diseases; Nov2023, Vol. 29 Issue 8, p3381-3392, 12p
Abstrakt:	Objective: To investigate the relationship between the polymorphisms rs1544410 (BsmI), rs2228570 (FokI) and rs731236 (TaqI) and DNA methylation status in the VDR gene (vitamin D receptor) with oral mucositis (OM) in oncopaediatric patients treated with methotrexate (MTX®). Methods: The population comprised healthy patients with haematological malignancies aged between 5 and 19 years. An evaluation of oral conditions was performed using the Oral Assessment Guide. Demographic, clinical, biochemical and haematological data were obtained from medical records. Genomic DNA from oral mucosal cells was used for the analysis of polymorphisms (n = 102) (PCR‐restriction fragment length polymorphism) and DNA methylation (n = 81) (methylation‐specific PCR). Results: Males predominated (57.8%), and the mean age was 10.3 years (±4.7). OM affected 84.3% of patients, of which 53.1% developed severe oral mucositis (SOM). Patients with OM had lower platelet and leukocyte counts (p < 0.05). The G allele of rs1544410 (p = 0.040) and the CT genotype of rs2228570 polymorphisms were associated with SOM (p = 0.038). A partially methylated status in the VDR promoter was found in all patients. Conclusion: OM is associated with lower leukocyte and platelet counts. SOM is associated with the rs1544410 and rs2228570 polymorphisms. The methylation status of the VDR is not associated with inflammation or exposure to MTX®. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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