| Autor: |
Wang, Jing, Xiao, Bing, Zhang, Ruiying, Guan, Haitao, Yang, Yang, Ding, Yijia, Yuan, Ran, Zhang, Xiaolei, Li, Lingyan, Liang, Jingang, Wen, Hongtao |
| Předmět: |
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| Zdroj: |
Journal fuer Verbraucherschutz und Lebensmittelsicherheit; Dec2023, Vol. 18 Issue 4, p451-460, 10p |
| Abstrakt: |
DBN9501 is a new maize transgenic event characterised by being resistance to insects and herbicides. To meet genetically modified (GM)-labeling requirements and monitor the unintended release of genetically modified organisms (GMOs), developing a creditable and applicable method for identifying and quantifying GM events is essential. Herein we developed an event-specific method and validated it through inter-laboratory ring trials using blind samples. The limit of detection (LOD) and limit of quantification (LOQ) of copy number ratio were confirmed at 0.05% and 0.1%, respectively. The quantitative bias ranged from -3.52 to 10.38%, and the relative standard deviation (RSD) of the method was < 25%. Furthermore, the expanded uncertainty for the blind samples S1 − S5 was 0.22%, 0.10%, 0.05%, 0.03%, and 0.02%. These results demonstrated that the event-specific quantitative PCR method could identify and quantify GM DBN9501 for further routine lab analysis. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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