| Autor: |
Wang, Xing, Qin, Jie, Xiang, Guoxiu, Wang, Chen, Wang, Qichen, Qin, Juanxiu, Wang, Haiying, Shen, Zhen |
| Předmět: |
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| Zdroj: |
Journal of Antimicrobial Chemotherapy (JAC); Dec2023, Vol. 78 Issue 12, p2890-2894, 5p |
| Abstrakt: |
Objectives To determine the genomic features of IMP-4-producing Klebsiella pneumoniae isolates recovered from paediatric patients and the transmission dynamics of bla IMP-4. Methods IMP-producing K. pneumoniae isolates were collected from paediatric patients in Shanghai Children's Medical Center from 2013 to 2020. WGS was performed for all isolates, and the complete genomes of three IMP-4-producing isolates were generated. The distribution of bla IMP-4-harbouring plasmids was determined, and a conjugation assay was employed to investigate the horizontal transfer of bla IMP-4-harbouring plasmids. Results We collected 21 bla IMP-carrying K. pneumoniae isolates, with IMP-4 (16/21, 76.2%) as the predominant subtype, followed by IMP-8 (n = 3) and IMP-26 (n = 2). IMP-4-producing isolates displayed a diverse population structure and all bla IMP-4 genes were located on plasmids, including IncN (n = 9), IncHI5 (n = 5), IncFII(K) (n = 1) and IncFII(pKP91) (n = 1), although only IncN plasmids were conjugative. Clonal transmission of ST101 strains carrying IncHI5 bla IMP-4-harbouring plasmids was observed, and the acquisition of bla IMP-4 by the international high-risk ST101 clone constituted a novel combination of ST101 clone and carbapenemase genes. Plasmid analysis demonstrated that the conjugal transfer of the IncHI5 bla IMP-4-harbouring plasmid might be blocked by the ST101 bacterial host. Conclusions The horizontal transfer of IncN plasmids and clonal spread of the international high-risk ST101 clone facilitated the nosocomial dissemination of bla IMP-4 among K. pneumoniae. The emerging IMP-4-producing ST101 clone displays diverse combinations of carbapenemase genes, and this clone could be a continually evolving threat and warrants prospective monitoring. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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