| Autor: |
Xia, Han, Zhang, Zhe, Luo, Chen, Wei, Kangfei, Li, Xuming, Mu, Xiyu, Duan, Meilin, Zhu, Chuanlong, Jin, Luyi, He, Xiaoqing, Tang, Lingjie, Hu, Long, Guan, Yuanlin, Lam, David C. C., Yang, Junbo |
| Předmět: |
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| Zdroj: |
iMeta; Nov2023, Vol. 2 Issue 4, p1-16, 16p |
| Abstrakt: |
We present multiPrime, a novel tool that automatically designs minimal primer sets for targeted next‐generation sequencing, tailored to specific microbiomes or genes. MultiPrime enhances primer coverage by designing primers with mismatch tolerance and ensures both high compatibility and specificity. We evaluated the performance of multiPrime using a data set of 43,016 sequences from eight viruses. Our results demonstrated that multiPrime outperformed conventional tools, and the primer set designed by multiPrime successfully amplified the target amplicons. Furthermore, we expanded the application of multiPrime to 30 types of viruses and validated the work efficacy of multiPrime‐designed primers in 80 clinical specimens. The subsequent sequencing outcomes from these primers indicated a sensitivity of 94% and a specificity of 89%. Highlights: MultiPrime is a user‐friendly and one‐step tool for designing targeted next‐generation sequencing primer sets.It integrates degenerate primer design theory with mismatch handling, resulting in improved accuracy and specificity in detecting broad‐spectrum sequences.It outperformed conventional programs in terms of run time, primer number, and primer coverage.The versatility and potential of multiPrime are highlighted by its potential application in detecting single or multiple genes, exons, antisense strands, RNA, or other specific DNA segments. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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