| Autor: |
Torkzadeh, Tahoura, Asadi, Zahra, Jafari Atrabi, Mohammad, Eivazkhani, Farideh, Khodadi, Maryam, Hajiaghalou, Samira, Akbarinejad, Vahid, Fathi, Rouhollah |
| Předmět: |
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| Zdroj: |
Reproduction, Fertility & Development; 2023, Vol. 35 Issue 17/18, p733-749, 17p |
| Abstrakt: |
Context: Base medium containing knock-out serum replacement (KSR) has been found to support formation and maintenance of follicles in one-day-old mice ovaries, but has not been shown to properly support activation and growth of primordial follicles. Aims: The present study was conducted to tailor the hormonal content of base medium containing KSR to enhance development of primordial follicles in neonatal ovaries. Methods: One-day-old mice ovaries were initially cultured with base medium for four days, and then, different hormonal treatments were added to the culture media and the culture was proceeded for four additional days until day eight. Ovaries were collected for histological and molecular assessments on days four and eight. Key results: In experiment I, the main and interactive effects of FSH and testosterone were investigated and FSH promoted activation of primordial follicles and development of primary and preantral follicles, and upregulated genes of phosphoinositide 3-kinase (Pi3k), KIT ligand (Kitl), growth differentiation factor 9 (Gdf9) and follicle stimulating hormone receptor (Fshr) (P < 0.05). Combination of testosterone and FSH, moreover, boosted gene expression of bone morphogenetic protein 15 (Bmp15), Connexin-43 (Cx43) and luteinising hormone and choriogonadotropin receptor (Lhcgr) (P < 0.05). In experiment II, the effect of various gonadotropins including FSH, equine chorionic gonadotropin (eCG) and human menopausal gonadotropin (hMG) on cultured ovaries was assessed and hMG supported development of primary follicles greater than eCG and FSH did (P < 0.05). Additionally, eCG and hMG increased gene expression of Lhcgr (P < 0.05), but FSH did not (P > 0.05). Conclusions: Supplementation of culture medium containing KSR with gonadotropins, particularly hMG, could improve follicular growth and expression of factors regulating follicular development. Implications: This study was a step forward in formulating an optimal medium for development of follicles in cultured one-day-old mice ovaries. Flowchart showing the procedure for isolating cultured ovaries for studying follicular development. Flowchart showing the procedure for isolating cultured ovaries for studying follicular development. Optimisation of medium for culture of one-day-old mice ovaries is of importance as it could serve as a model for studying the follicular growth and dynamics under in vitro condition and developing alternative assisted reproductive techniques for preservation of fertility in cancer patients and conservation of endangered animal species. In the current research, we examined the effect of various hormonal treatments on follicular development and expression of the corresponding factors and concluded that incorporation of gonadotropins, particularly hMG, in culture medium could improve follicular growth and expression of factors regulating follicular development in one-day-old mice ovaries. Image by Zahra Asadi. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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