Screening of Poly-β-hydroxybutyrate (PHB) Producing Bacteria and yeasts from locally vendored 'Whey' - a biowaste sample.

Autor: Zisha, Shishita Jahan, Mumtaz, Tabassum, Alam, A. K. M. Rashidul
Předmět:
Zdroj: Biological Environment & Pollution; 2022, Vol. 2 Issue 1, p7-15, 9p
Abstrakt: Whey- a byproduct of cheese manufacture, has several commercial uses. The present study explored the microbial load of the whey and the possible existence of biopolymer (PHB) producing microorganisms in this byproduct. Whey samples collected from different vendors in the locality of Mirpur, Dhaka, were brought into the laboratory, and the microbial load of each sample was determined by serial dilution technique using nutrient agar and YEPD medium. From 30 colonies, 32 strains were preliminarily isolated. Out of 32 isolates, fifteen exhibited pink fluorescence on mineral medium, indicating the presence of PHB production inside cells. Among these, seven strains were identified as members of the genera Candida tropicalis; based on molecular characterization. The remaining 8 isolates were bacteria. Yeast isolates were obtained from locally vendored whey samples, whereas bacterial strains were predominant in commercial samples. This work can be considered the first step in developing an eco-friendly bio-refinery pathway for the cheese industry. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index