| Abstrakt: |
[Objective] The structure and function of yak heart fatty acid binding protein 3 (FABP3) gene were analyzed and its expression levels in adult and fetal yak were detected to provide references for xploring biological functions of the gene in yak breeding. [Method] The FABP3 gene was amplified by PCR from heart tissue of Meiren yak, and bioinformatics analysis was carried out after the CDS region was obtained. The expression levels of FABP3 in heart, liver, spleen, lung, kidney, and muscle tissues of adult and fetal yak were detected by real-time quantitative PCR (RT-qPCR). [Result] The length of the coding egion of FABP3 was 402 bp, encoding 133 amino acids. The advanced structure of the protein included mainly β-turn and extended chain. There was no transmembrane region or signal peptide on yak FABP3 protein, indicating it was a stable protein with certain hydrophilicity. Homology analysis of the nucleotide and amino acid sequences of the CDS region showed that the FABP3 gene was conserved in cattle. Accordng to the phylogenetic tree, yak had the closest genetic relationship with wild yak and zebu, and the farhest genetic relationship with chicken. RT-qPCR results showed that the FABP3 gene was expressed in heart, liver, spleen, lung, kidney, and muscle tissues of adult and fetal yak. The expression in liver, spleen, kidney, and muscle of fetal cattle was significantly or extremely significantly higher than that of adult yak, and the expression level in lung of adult yak was significantly higher than that of fetal yak. [Conclusion] The FABP3 gene of yak was cloned, and its tissue expression pattern was explored. This study provided asic data for further study on the role of FABP3 in fat deposition. [ABSTRACT FROM AUTHOR] |
