| Autor: |
Colombo, Michele, Grauso, Laura, Lanzotti, Virginia, Incerti, Guido, Adamo, Adele, Storlazzi, Aurora, Gigliotti, Silvia, Mazzoleni, Stefano |
| Předmět: |
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| Zdroj: |
Biology (2079-7737); Nov2023, Vol. 12 Issue 11, p1378, 19p |
| Abstrakt: |
Simple Summary: The article describes the inhibitory effects of self-DNA in the insect Drosophila melanogaster. The larvae fed with yeast containing their self-DNA showed a significant delay in their development and some increased mortality. The chemical analysis of these larvae showed changes of their metabolites' composition, with the presence of some compounds known for their effects on insect development and/or fertility. In fact, the adult flies grown from self-DNA-treated larvae showed a strongly reduced egg deposition. We investigated the effects of dietary delivered self-DNA in the model insect Drosophila melanogaster. Self-DNA administration resulted in low but significant lethality in Drosophila larvae and considerably extended the fly developmental time. This was characterized by the abnormal persistence of the larvae in the L2 and L3 stages, which largely accounted for the average 72 h delay observed in pupariation, as compared to controls. In addition, self-DNA exposure affected adult reproduction by markedly reducing both female fecundity and fertility, further demonstrating its impact on Drosophila developmental processes. The effects on the metabolites of D. melanogaster larvae after exposure to self-DNA were studied by NMR, LC-MS, and molecular networking. The results showed that self-DNA feeding reduces the amounts of all metabolites, particularly amino acids and N-acyl amino acids, which are known to act as lipid signal mediators. An increasing amount of phloroglucinol was found after self-DNA exposure and correlated to developmental delay and egg-laying suppression. Pidolate, a known intermediate in the γ-glutamyl cycle, also increased after exposure to self-DNA and correlated to the block of insect oogenesis. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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