| Abstrakt: |
When rawhide fleshing (140–230 kg/ton) wastes from beam houses are disposed of, it substantially negatively influences the environment. Hence, we focus on isolating and characterizing a commercially valuable component, collagen, from tannery wastes and demonstrating its in vitro cytotoxicity and proliferative activity for its biomedical applications. After an alkaline pre-treatment, the extraction process involved treating the fleshing with acid and enzyme. The extracted collagen was further subjected to structural and thermal characterization. The in vitro cytotoxicity and proliferation assay was studied using a 3T3-L1 fibroblast cell line. The percentage yield of pepsin-soluble collagen (PSC) was higher than that of acid-soluble collagen (ASC), 22.16 ± 1.25% and 12.93 ± 1.04%, respectively. Fourier transform infrared (FTIR) spectrum confirmed the presence of amides A, B, I, II, and III with the functional group of N-H, CH2, C=O, C-N, and N-H. The electrophoresis pattern and amino acids analysis showed that the collagen was in its native form. The isolated collagen possessed the native structural integrity which was confirmed by the result of X-ray diffraction (XRD) and differential scanning calorimetry (DSC) studies. The thermal denaturation temperature was at 38 °C for PSC. The morphological study revealed that both ASC and PSC exhibited fibrous structure. In vitro biocompatibility tests revealed that the isolated collagen did not cause significant cytotoxicity, and there was no detectable cell death or growth inhibition. From the above findings substantiate the application of hide waste-derived collagen as a safe and cost-effective substitute in various pharmaceutical and biomedical domains. [ABSTRACT FROM AUTHOR] |
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