| Autor: |
Lejla, Ušanović, Jasna, Hanjalić Kurtović, Lejla, Lasić, Adnan, Čučuković, Dalila, Destanović, Lejla, Smailagić Vesnić, Merima, Miralem, Belma, Kalamujić Stroil |
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| Zdroj: |
Genetics & Applications; 2023 Special Issue, p43-43, 1p |
| Abstrakt: |
The Balkan peninsula is widely known for its high biodiversity and endemism in freshwater ecosystems, with aquatic insects being the most recognizable group of macroinvertebrates. Caddisflies (Trichoptera) have been extensively studied since the late 19th century, and new species and subspecies are still being discovered and described. DNA barcoding is a widely used tool in biodiversity research and it is dependent on the availability of reference genetic sequence libraries. We collected larvae samples of aquatic insects and performed DNA barcoding using degenerated primers LCO1490-JJ and HCO2198-JJ. The sequence data for standard animal DNA barcode of specimens collected in two protected natural areas of the Sarajevo city's urban area, Dariva (river Miljacka) and Vrelo Bosne (river Bosna), were stored in the GenBank database. Based on our data a new subspecies Rhyacophila fasciata delici was discovered in the investigated areas (Valladolid et al. 2020). Among the nine GenBank enteries for this subspecies that we included in the database (accession numbers: MT765286, MT765287, MT765290, MT772015, MT772017, MT772020, MT772022, MT772028, MT772029), there are five different haplotypes. Three haplotypes were recorded for Dariva and two haplotypes for the Vrelo Bosne locality. The recorded subspecies and different haplotypes within it indicate that the diversity of Trichoptera species in Bosnia and Herzegovina is far from being fully explored. The use of DNA barcoding, in conjunction with morphological species identification, should be employed in the biomonitoring of protected areas in Canton Sarajevo. Our study once again emphasizes the importance of the existence and supplementation of publicly accessible genetic databases to improve species identification and bioassessment reliability. Further research with a larger sample size and in a wider area should shed more light on aquatic insects and their diversity in protected areas, not only in Canton Sarajevo but also throughout Bosnia and Herzegovina. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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