| Abstrakt: |
Elaeocarpus ganitrus (Rudraksha) contains many bioactive compounds with potent antioxidant and pharmacological benefits. This study designed to identify the bioactive compounds in the three extracts prepared from E. ganitrus leaves using UV-Vis and FTIR spectroscopy and evaluate their antioxidant potential. The preliminary phytochemical screening showed that all three extracts are rich in alkaloids, flavonoids, phenolics, tannins, and saponins. The UV-Vis analysis displayed two distinct peaks at 212.6 nm and 275 nm in the absorption range of 0.9 - 1.0 with hydroalcoholic extract (EGHA), 0.4 - 0.5 with ethanolic extract (EGE), and 0.3 - 0.4 with water extract (EGW), respectively. The FT-IR spectrum showed the presence of alkene, amine and fluoro compound, alkyl aryl ether, alkane, ester, δ-lactone, cyclopentanone, amine salt, alcohol, and carboxylic acid. All three extracts of E. ganitrus leaves have been subjected to the assessment of antioxidant properties by using 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) and Nitric oxide (NO) scavenging radical scavenging assays. The results revealed that hydroalcoholic extract (EGHA) displayed better antioxidant potential as compared to ethanolic extract (EGE) and water extract (EGW) in all tested concentrations. This study demonstrates the significance of the FTIR and UV-Vis spectrum of E. ganitrus, which can be exploited to isolate significant phytoconstituents. Furthermore, biologically active isolates can be analysed to investigate their diverse biological activities depending on their therapeutic applications in the pharmaceutical industries. [ABSTRACT FROM AUTHOR] |
