| Autor: |
Tamassia, Nicola, Bianchetto-Aguilera, Francisco, Gasperini, Sara, Grimaldi, Alessio, Montaldo, Claudia, Calzetti, Federica, Gardiman, Elisa, Signoretto, Ilaria, Castellucci, Monica, Barnaba, Vincenzo, Tripodi, Marco, Cassatella, Marco Antonio |
| Předmět: |
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| Zdroj: |
Frontiers in Immunology; 2023, p1-16, 16p |
| Abstrakt: |
Introduction: Peripheral monocytes in humans are conventionally divided into classical (CL, CD14++CD16− ), intermediate (INT, CD14++CD16+) and nonclassical (NC, CD14dim/− CD16++) cells, based on their expression levels of CD14 and CD16. A major fraction of the NC-monocytes has been shown to express the 6-sulfo LacNAc (slan) antigen, but whether these slan+/NCmonocytes represent the prototypical non-classical monocytes or whether they are simply a sub-fraction with identical features as the remainder of NC monocytes is still unclear. Methods: We analyzed transcriptome (by bulk and single cell RNA-seq), proteome, cell surface markers and production of discrete cytokines by peripheral slan+/NC- and slan− /NC-monocytes, in comparison to total NC-, CL- and INT- monocytes. Results: By bulk RNA-seq and proteomic analysis, we found that slan+/NCmonocytes express higher levels of genes and proteins specific of NCmonocytes than slan− /NC-monocytes do. Unsupervised clustering of scRNAseq data generated one cluster of NC- and one of INT-monocytes, where all slan+/NC-monocytes were allocated to the NC-monocyte cluster, while slan− / NC-monocytes were found, in part (13.4%), within the INT-monocyte cluster. In addition, total NC- and slan− /NC-monocytes, but not slan+/NC-monocytes, were found by both bulk RNA-seq and scRNA-seq to contain a small percentage of natural killer cells. Conclusion: In addition to comparatively characterize total NC-, slan− /NC- and slan+/NC-monocyte transcriptomes and proteomes, our data prove that slan+/ NC-, but not slan− /NC-, monocytes are more representative of prototypical NC-monocytes. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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