Identification of Differential Metabolites Between Type 2 Diabetes and Postchronic Pancreatitis Diabetes (Type 3c) Based on an Untargeted Metabolomics Approach.
| Autor: | Qi, Liang, Ye, Zheng, Lin, Hao |
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| Předmět: |
DIAGNOSIS of diabetes
BIOMARKERS GLYCOLS CARNITINE ACADEMIC medical centers CONFIDENCE intervals CHRONIC diseases METABOLOMICS LIQUID chromatography DIABETES DIFFERENTIAL diagnosis METABOLISM BLOOD collection MANN Whitney U Test TYPE 2 diabetes T-test (Statistics) COMPARATIVE studies RESEARCH funding MASS spectrometry BILE acids CARBOXYLIC acids DESCRIPTIVE statistics CHI-squared test PANCREATITIS RECEIVER operating characteristic curves STATISTICAL correlation DATA analysis software METABOLITES LIPIDS FATTY acids SPHINGOLIPIDS DISEASE complications BLOOD |
| Zdroj: | Laboratory Medicine; Nov2023, Vol. 54 Issue 6, p562-573, 12p |
| Abstrakt: | Objective A nontargeted metabolomics approach was established to characterize serum metabolic profile in type 3c diabetes mellitus (T3cDM) secondary to chronic pancreatitis and compare with T2DM. Methods Forty patients were recruited for metabolite analysis based on liquid chromatography-mass spectrometry. Cluster heatmap and KEGG metabolic pathway enrichment analysis were used to analyze the specific and differential metabolites. The receiver operating characteristics (ROCs) were generated and correlation analysis with clinical data was conducted. Results Metabolites including sphingosine, lipids, carnitine, bile acid, and hippuric acid were found to be different between T2DM and T3cDM, mainly enriched in bile acid biosynthesis, fatty acid biosynthesis, and sphingolipid metabolic pathways. The ROCs were generated with an area under the curve of 0.907 (95% confidence interval, 0.726–1) for the model with 15 metabolites. Conclusion T3cDM is characterized by increased sphingosine, carnitine, bile acid, and most lipids, providing novel biomarkers for clinical diagnosis and a future direction in research on pathophysiological mechanisms. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
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