| Abstrakt: |
Baccaurea motleyana Mull. Arg (Baccaurea) is a tall plant whose fruit is consumed as food in Indonesia. This study aims to evaluate the antioxidant activity of the B. motleyana extracts and their antiproliferative effect on PANC-1 cancer cells. The ethanolic (EBM), ethyl acetate (EABM), and n-hexane (HBM) extracts of B. motleyana leaf were evaluated for their in vitro antiproliferative activity on pancreas cancer (PANC-1) cell lines. Mechanisms of extracts through p53 and Bcl-2 proteins expression using immunocytochemistry. Cell viability assays were performed by calculating the percentage of viable cells and used to determine the IC50 of extracts at 72 h. Spectrophotometrically measuring its antioxidant DPPH and ABTS radical scavenging activities. The phytochemicals profile of extracts was carried out using qualitative tests to identify alkaloids, flavonoids, saponins, triterpenoids/steroids, and tannins. Differences were considered as statistically significant at p<0.05 according to one way ANOVA test followed by Tukey's test. EBM contains more phytochemicals compound than EABM and HBM. The IC50 of EBM, EABM, and HBM were 41.36±1.79 μg/mL, 62.02±2.52 μg/mL, and 124.33±6.04 μg/mL, respectively (DPPH method, p<0.05). In addition, 34.07±2.51 μg/ml, 89.33±4.21 μg/ml, and 202.53±6.81 μg/ml, respectively (ABTS method, p<0.05). The extracts can inhibit the proliferation of PANC-1 cells at 24 h and 72 h. EBM has the strongest IC50 compared to EABM and HBM was 20.75±2.13 μg/mL, 94.63±5.32 μg/mL, and 176±7.10 μg/mL, respectively (p<0.05). Furthermore, EBM could exhibit antiproliferation via modulating the p53 and Bcl-2. EBM, EABM, and HBM have antioxidant and antiproliferative activity against PANC-1 cells. [ABSTRACT FROM AUTHOR] |
