| Autor: |
Bhatiani, Ambika, Nigam, Nilam, Nigam, Sanjay, Afaq, Nashra, Yadav, Madhu, Bano, Firoza, Kumar, Deepak, Saxena, Priyanka, Hassan, Sahina, Nabi, Nusrat |
| Předmět: |
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| Zdroj: |
Journal of Cardiovascular Disease Research (Journal of Cardiovascular Disease Research); 2023, Vol. 14 Issue 10, p1694-1708, 15p |
| Abstrakt: |
Introduction: Persistent suppurative otitis media (CSOM) is a persistent inflammation of the middle ear and mastoid cavity that persists for more than two weeks. Pseudomonas aeruginosa is a common pathogen that causes CSOM. One of the best medications for treating Pseudomonas infections is carbapenem. The development of bacteria resistant to carbapenems is frequently linked to metallo-β lactamases. In an effort to stop and propagate resistance, identifying the strains that create MBLs can help guarantee that patients receive the best therapy possible. Aim and Objective: To study the molecular characterization of metallo-beta lactamase gene with special reference to blaIMP-1 gene in imipenem resistant Pseudomonas aeruginosa isolates from patients of chronic suppurative otitis media. Material and Methods: The present study was a cross sectional study carried out in the Department of Microbiology with collaboration with the ENT and the Pharmacology Department for a period of 1 year i.e between August 2022 to August 2023 at a tertiary care centre. A total of 352 patients clinically suspected cases for CSOM were studied. Swabs taken from discharging ears were sent for Gram's staining, culture and antibiotic sensitivity test was performed according to the CLSI guidelines 2022. The isolates were further tested for MBL by screening test, by Imipenem - EDTA combined disc test, and MBL E test (Imipenem). The DNA was extracted by using Qiagen DNA Extraction kit, which was further proceeded for the blaIMP-1 gene detection for Pseudomonas aeruginosa by the conventional PCR. Results: In the present study the clinically diagnosed suspected cases of CSOM were 352, out of which 118 (33.5%) was found to be positive for CSOM infection. The ratio of Male 74 (62.7%) was found to be more as compared to the Female 44 (37.2%), with the maximum cases in the age group of 0-10 and the least in the age group above 41 years. In our study it was observed that the maximum number of cases was observed in the Gram negative bacilli isolates (77.2%) as compared to the Gram positive isolates (19.4%) with sides of the ear almost equally affected. P. aeruginosa 58 (49.1%) was the most common isolate followed by Klebsiella spp with 19 (16.1%) and among gram positive isolate Staphylococcus aureus was found to be 15.2%, there was only 3 case found for candida albicans (2.5%). The sensitivity observed for P. aeruginosa for Colistin was (96.5%), Piperacillin-tazobactam (74.1%), Amikacin (77.5%), and Cefipime (74.1%) were found to be the most effective Antibiotics. The resistance to ciprofloxacin was (58.6%), Levofloxacin (50%), Piperacillin(25.8%), Gentamicin (36.2%), Imipenem (36.2%), Tobramycin and Ceftazidime with 27.5% respectively. The molecular characterization of the blaIMP-1 gene was detected in 18 (31%) of the isolates of Pseudomonas aeruginosa which were screening test-positives for MBL by Imipenem - EDTA combined disc test, and MBL E test (Imipenem). Conclusion: For a treatment to be effective and to prevent both medical problems and antibiotic resistance, it is essential to understand the etiological agents of CSOM and their antibiogram. It is imperative that wide spectrum antibiotics like imipenem be used for the need of the hour. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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