Autor: |
SHINGO MIYAMOTO, TOYOFUMI HIRAKAWA, YUKIKO NOGUCHI, DAICHI URUSHIYAMA, KOHEI MIYATA, TSUKASA BABA, FUSANORI YOTSUMOTO, SHIN'ICHIRO YASUNAGA, KAZUHIKO NAKABAYASHI, KENICHIRO HATA, WATARU NAKAGAWA, TOSHIHIRO OTSUKA, YOSHIKO NOZAWA, IPPEI FURUHATA, JITSUO MIKASA |
Předmět: |
|
Zdroj: |
In Vivo; Nov/Dec2023, Vol. 37 Issue 6, p2555-2563, 9p |
Abstrakt: |
Background/Aim: Ultrafine bubbles (UFBs) have been extensively researched owing to their promising physical and biological properties. However, determining the lifespan or ideal concentration of UFBs for various biological events is challenging. This study aimed to determine the maximum concentration and longest lifespan of UFBs and to verify the validity of UFBs for assessing cell properties. Materials and Methods: A generator system (HMB-H0150+P001, TOSSLEC Corporation Limited, Kyoto, Japan) generated UFBs using various gases. The size and concentration of UFBs in ultrapure water and cell culture medium were measured through a nanoparticle tracking analysis method. Results: The UFB concentration increased when the generator operated in a time dependent manner. The mean size of UFBs [ABSTRACT FROM AUTHOR] |
Databáze: |
Complementary Index |
Externí odkaz: |
|