| Autor: |
Brom, Julia A., Samsri, Sasiprapa, Petrikis, Ruta G., Parnham, Stuart, Pielak, Gary J. |
| Zdroj: |
Biomolecular NMR Assignments; Dec2023, Vol. 17 Issue 2, p235-238, 4p |
| Abstrakt: |
Adenylate kinase reversibly catalyzes the conversion of ATP plus AMP to two ADPs. This essential catalyst is present in every cell, and the Escherichia coli protein is often employed as a model enzyme. Our aim is to use the E. coli enzyme to understand dry protein structure and protection. Here, we report the expression, purification, steady-state assay, NMR conditions and 1H, 13C, 15N backbone resonance NMR assignments of its C77S variant. These data will also help others utilize this prototypical enzyme. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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