RuX: A Novel, Flexible, and Sensitive Mifepristone-Induced Transcriptional Regulation System.

Autor:	Meinzinger, Anne, Zsigmond, Áron, Horváth, Péter, Kellenberger, Alexandra, Paréj, Katalin, Tallone, Tiziano, Flachner, Beáta, Cserhalmi, Marcell, Lőrincz, Zsolt, Cseh, Sándor, Shmerling, Doron
Předmět:	GENETIC transcription regulation GLUCOCORTICOID receptors GENETIC regulation CELL culture RECOMBINASES
Zdroj:	International Journal of Cell Biology; 10/31/2023, p1-12, 12p
Abstrakt:	Inducible gene regulation methods are indispensable in diverse biological applications, yet many of them have severe limitations in their applicability. These include inducer toxicity, a limited variety of organisms the given system can be used in, and side effects of the induction method. In this study, a novel inducible system, the RuX system, was created using a mutant ligand-binding domain of the glucocorticoid receptor (CS1/CD), used together with various genetic elements such as the Gal4 DNA-binding domain or Cre recombinase. The RuX system is shown to be capable of over 1000-fold inducibility, has flexible applications, and is offered for use in cell cultures. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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