| Autor: |
Puzankova, Olga, Gavrilova, Vera, Chernyshev, Roman, Kolbin, Ivan, Igolkin, Alexey, Sprygin, Alexandr, Chvala, Ilya, Mazloum, Ali |
| Předmět: |
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| Zdroj: |
Methods & Protocols; Oct2023, Vol. 6 Issue 5, p73, 9p |
| Abstrakt: |
Isolation of African swine fever virus (ASFV) is a critical step towards the identification, titration, characterization, and even modification of the virus. Therefore, it is important to identify a suitable cell line that supports the efficient replication of ASFV for these purposes. This should be achieved even when starting with a low virus load, as in the case of isolating the virus from field samples. This article presents a detailed protocol on the preparation of porcine bone marrow primary (PBMP) cell culture, which has a high sensitivity towards ASFV, resulting in high viral yields with a minimal risk of bacterial contamination. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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