| Abstrakt: |
Gentamicin (GEN), a widely used antibiotic, is known to induce nephrotoxicity. Raspberry ketone (RK), a natural compound found in red raspberries has demonstrated potential protective effects against various pathological conditions. This study aimed to evaluate the protective effect of raspberry ketone in gentamicin-induced nephrotoxicity in Wistar rats. Twentyfour Wistar rats were randomly divided into four groups: normal control group; rats received 0.5ml normal saline for 14 days i.p., diseased control group(GEN); rats received GEN (100 mg/kg,i.p.) for 7 consecutive days. GEN+ RK1: rats received GEN (100 mg/kg/) i.p. for 7 consecutive days + raspberry ketone (RK) (100 mg/kg/day) p.o. for 14 days. GEN+ RK2: rats received gentamicin (100 mg/kg/) i.p. for 7 consecutive days + RK (200 mg/kg/day) p.o. for 14 days At the end of the treatment period urine samples were collected from metabolic cages, animal were anaesthetized and blood and kidney tissue samples were collected for biochemical and histopathological evaluations. The gentamicin treated group showed significant increased in oxidative stress manifested by decrease in GSH, SOD, CAT and increased in TBARS level. Further, increased in serum creatinine, albumin and blood urea nitrogen levels compared to the control group, indicating renal dysfunction. However, the administration of raspberry ketone in gentamicin treated mice significantly lowers the levels of these markers compared to the disease control group, suggesting a protective effect of raspberry ketone against gentamicin-induced nephrotoxicity. The histopathological analysis also revealed severe tubular necrosis and interstitial inflammation in the gentamicin treated group, whereas the raspberry ketone treated groups showed reversal in these changes. In conclusion, raspberry ketone supplementation attenuated renal dysfunction by modulating oxidative stress parameters, creatinine, albumin and blood urea nitrogen levels and reduced histopathological changes associated with gentamicin toxicity. [ABSTRACT FROM AUTHOR] |
