| Abstrakt: |
The fungus Trametes versicolor, a basidiomycete, is well recognized as a source of industrial enzymes including laccase. Laccase productivity of T. versicolor NFCCI 4361 was determined at different concentrations of molasses, copper (Cu), guaiacol (Gua), gallic acid (GA), and 2, 2´-azino bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) in Potato Dextrose Broth (PDB). Copper with molasses synergistically increased the laccase productivity of T. versicolor NFCCI 4361 from 291.59 ± 30.92 to 1228.96 ± 87.52 U/L. The GA and Gua had negligible effects, while ABTS (1 mM) greatly increased the laccase productivity, both on its alone (495.4 ± 83.47 U/L) and when combined with copper (921.71 ± 41.58 U/L). Following the study of laccase productivity, the percentage of spent wash decolorization was determined by using powdered activated carbon (AC), living, dead, and activated carbon immobilized mycelial biomass (ACIMB) of T. versicolor NFCCI 4361, comparatively. The maximum percentage of decolorization was obtained by ACIMB (44%), followed by AC (40%), living (30%), and dead (16%) mycelial biomass of T. versicolor NFCCI 4361 after 144 h of interaction time. [ABSTRACT FROM AUTHOR] |
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