| Autor: |
Sapozhnikova, Yulia P., Koroleva, Anastasia G., Yakhnenko, Vera M., Volkova, Aleksandra A., Avezova, Tatyana N., Glyzina, Olga Yu., Sakirko, Mariya V., Tolstikova, Lyubov I., Sukhanova, Lyubov V. |
| Předmět: |
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| Zdroj: |
Biology (2079-7737); Oct2023, Vol. 12 Issue 10, p1348, 26p |
| Abstrakt: |
Simple Summary: Temperature fluctuations can affect the overall stability of the biomolecules involved in biological processes in ectothermic species, which can alter the overall plasticity of the species and is manifested in their accelerated cellular aging. The aim of this work was to evaluate the effects of thermal pre-adaptation/preconditioning on whitefish forms, which holds potential for aquaculture, particularly on their stress tolerance and stress-induced senescence with the use of key cellular and molecular biomarkers: the volume of functionally active mitochondria, telomerase activity, and telomere length. We concluded that thermal preconditioning appears to be highly effective in preventing telomere attrition in cold-water whitefish, and may play a preventive role in the development of stress-resistant aquaculture. The proposed biomarkers could be used at earlier stages of ontogeny as indicators of juvenile fish's well-being and plasticity in aquaculture or the natural environment due to climate change. One of the little-studied ways that climate warming or temperature increases in aquaculture could affect aquatic animals is through accelerated aging. This study is dedicated to understanding the principles of molecular and cellular aging in the target tissues of juvenile whitefishes (Yenisei hump-snout whitefish and its hybrid) under the influence of acute heat stress (up to 26 °C), and the effects of thermal preconditioning as pre-adaptation. Non-adapted stressed hump-snout whitefish showed a higher induction threshold for functionally active mitochondria in the blood and a decrease in telomerase activity in the liver after heat shock exposure as a long-term compensatory response to prevent telomere shortening. However, we observed heat-induced telomere shortening in non-adapted hybrids, which can be explained by a decrease in mitochondrial membrane stability and a gradual increase in energy demand, leading to a decrease in protective telomerase activity. The pre-adapted groups of hump-snout whitefish and hybrids showed a long-term or delayed response of telomerase activity to heat shock, which served as a therapeutic mechanism against telomere shortening. We concluded that the telomerase and telomere responses to thermal stress demonstrate plasticity of tolerance limits and greater stability in hump-snout whitefish compared with hybrids. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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