| Autor: |
Martínez Barreiro, Mariana, Vázquez Alberdi, Lucia, De León, Lucila, Avellanal, Guadalupe, Duarte, Andrea, Anzibar Fialho, Maximiliano, Baranger, Jérôme, Calero, Miguel, Rubido, Nicolás, Tanter, Mickael, Negreira, Carlos, Brum, Javier, Damián, Juan Pablo, Kun, Alejandra |
| Předmět: |
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| Zdroj: |
Biology (2079-7737); Oct2023, Vol. 12 Issue 10, p1324, 15p |
| Abstrakt: |
Simple Summary: In this work, we explore the central compromise in TrJ/+ mice, a model for the peripheral neuropathy Charcot-Marie-Tooth, using three different approximations: Ultrafast Doppler, Confocal Microscopy, and behavioral tests, exposing alterations in the brain vasculature, as well as an anxiety-like behavior. Hemodynamic changes recorded in vivo, associated with vascular volume modulation, together with behavioral alterations in the TrJ/+ model, account for a functional-structural-behavioral profile that demonstrates vascular/central involvement of the disease. The main human hereditary peripheral neuropathy (Charcot-Marie-Tooth, CMT), manifests in progressive sensory and motor deficits. Mutations in the compact myelin protein gene pmp22 cause more than 50% of all CMTs. CMT1E is a subtype of CMT1 myelinopathy carrying micro-mutations in pmp22. The Trembler-J mice have a spontaneous mutation in pmp22 identical to that present in CMT1E human patients. PMP22 is mainly (but not exclusively) expressed in Schwann cells. Some studies have found the presence of pmp22 together with some anomalies in the CNS of CMT patients. Recently, we identified the presence of higher hippocampal pmp22 expression and elevated levels of anxious behavior in TrJ/+ compared to those observed in wt. In the present paper, we delve deeper into the central expression of the neuropathy modeled in Trembler-J analyzing in vivo the cerebrovascular component by Ultrafast Doppler, exploring the vascular structure by scanning laser confocal microscopy, and analyzing the behavioral profile by anxiety and motor difficulty tests. We have found that TrJ/+ hippocampi have increased blood flow and a higher vessel volume compared with the wild type. Together with this, we found an anxiety-like profile in TrJ/+ and the motor difficulties described earlier. We demonstrate that there are specific cerebrovascular hemodynamics associated with a vascular structure and anxious behavior associated with the TrJ/+ clinical phenotype, a model of the human CMT1E disease. [ABSTRACT FROM AUTHOR] |
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