The combination effect of ultrasound and laser therapy on wound healing in diabetic rat model: histological and biomechanical evaluations.

Autor:	Ebrahiminia, Ali, Khanaki, Korosh, Ezzati, Kamran, Gazor, Rouhollah, Taeb, Shahram
Předmět:	BLOOD sugar analysis WOUND healing BIOLOGICAL models COLLAGEN STAINS & staining (Microscopy) ANIMAL experimentation DIABETES LASER therapy AMINOGLYCOSIDES RATS BACK RESEARCH funding TENSILE strength ULTRASONIC therapy COMBINED modality therapy BIOMECHANICS HISTOLOGY
Zdroj:	International Journal of Diabetes in Developing Countries; Dec2023, Vol. 43 Issue 6, p1015-1021, 7p
Abstrakt:	Background: Defect in wound healing is a serious problem in diabetes. There are several physical treatment approaches such as ultrasound and laser therapy. This study was designed to determine the combination effect of ultrasound and laser on diabetic wound healing in a rat model. Methods: Thirty Wistar rats were divided into five groups; healthy control, diabetic control, laser (wavelength of 905 nm), ultrasound (frequency of 3 MHz), and laser plus ultrasound for 5 days a week for 2 weeks. Diabetes was induced by injection of streptozotocin (55 mg/kg). Afterward, a 3-cm wound was made with an incision on the back of the animals. On the 14th day of the treatments, blood fasting glucose was determined by glucometer. Also, biomechanical and histological assessments of the tissues were performed by Van Gieson, trichrome stains, and tensiometry methods, respectively. Results: The collagen and elastin amounts were significantly decreased in diabetic control group in comparison to healthy group. The tensile parameters, collagen, and elastin amounts in diabetic rats under different treatments were remarkably increased than in diabetic control rats. Also, the combination of ultrasound and laser caused more increase in collagen and elastin levels as well as tissue tensile parameters than ultrasound or laser. Conclusion: It appears that ultrasound or laser intervention is effective in the wound healing of diabetes condition partly through increasing the amount of collagen and elastin and also in improving the biomechanical properties. In addition, the combination therapy of ultrasound and laser might be more effective than each one alone. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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