| Autor: |
Gawali, Poonam, Ramteke, Larkins, Jadhav, B. L., Khade, Bipin S. |
| Předmět: |
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| Zdroj: |
Journal of Cluster Science; Nov2023, Vol. 34 Issue 6, p2759-2779, 21p |
| Abstrakt: |
The present study portrays a rapid and eco-friendly method using ripe Sonneratia alba (S. alba) fruit extract (SF) as a bioreductant, stabilizer, and capping agent to synthesize gold nanoparticles (SF-AuNPs) with a size of 30 ± 0.2 nm. Fluorescence spectroscopy was used to investigate the biophysical interactions between SF-AuNPs and trypsin protein under various experimental parameters. Resonance light scattering analysis confirms the ground state interaction of SF-AuNPs-trypsin complex. Thermodynamic analysis reveals that SF-AuNPs- trypsin complex is mainly enthalpy and entropy-driven, the calculated free energy (ΔG° ranged from − 35.45 to − 23.87 kJ/mol−1). The adsorption of trypsin on SF-AuNPs was analyzed by Langmuir and Freundlich models. The circular dichroism (CD) data were performed to investigate the conformational alterations at the secondary structural level of SF-AuNPs-trypsin complex (α-helix 8.71, and β-sheet 38.47%). SF-AuNPs biological insights were investigated using several antioxidant and anti-inflammatory assays in vitro. Anticancer studies with SF-AuNPs on the lung cancer cell line (A549) were more effective than S. alba aqueous extract, the percent inhibition of AuNPs concentrations (6.25 nM) 4·33 ± 1.49% to (400 nM) 59·38 ± 0.92%. The present experimental data may provide new insights into the safe and practical applications of SF-AuNPs in biomedical areas. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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