| Abstrakt: |
Rapid industrialization leads to synthesis of many xenobiotic organic compounds, which has an adverse effect on the environment. The Rhodamine B is a xanthene class dye having a carcinogenic, mutagenic and fluorescent properties. Rhodamine B's increased toxicity causes developmental, neurological, skin-related, and many other forms of cancer even at low concentrations. This research assessed the microbial degradation of Rhodamine B through fungal cultures. The potent culture were screened and identified as Aspergillus fumigatus isolate P5. After the optimization with one factor at a time, 97.28% decolorization of Rhodamine B was obtained at pH 6.5, 25 °C with cellulose as carbon and diammonium hydrogen phosphate as nitrogen source with 5% inoculum at 10 ppm dye concentration. The statistical study was performed with response surface methodology followed Box–Behnken design. Four different parameters were selected and 28 combinations were tested for obtaining maximum Rhodamine B decolorization. Results found that the p-value < 0.05 means model was significant, and Rhodamine B concentration and pH were significant parameters for the research. The LC–MS analysis of metabolites reveals that Rhodamine B dye was degraded into N-diethyl-N′-ethyl rhodamine, N-ethyl rhodamine, butane 1,3-diol, propane 1,2,3-triol, hydroquinone and propionic acid by A. fumigatus isolate P5. As per the investigation, A. fumigatus have a potency to degrade a Rhodamine B efficiently. [ABSTRACT FROM AUTHOR] |
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