Comparative transcriptomic analysis of mammary gland tissues reveals the critical role of GPR110 in palmitic acid-stimulated milk protein and fat synthesis.
| Autor: | Zhang, Minghui, Ma, Zonghua, Qi, Hao, Cui, Xu, Li, Rui, Gao, Xuejun |
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| Předmět: |
METHIONINE metabolism
BIOLOGICAL models LACTATION PROTEINS SEQUENCE analysis ANIMAL experimentation CELL receptors PUBERTY MTOR inhibitors MILK proteins GENE expression profiling BREAST TISSUES CELL proliferation DESCRIPTIVE statistics RESEARCH funding POLYMERASE chain reaction EPITHELIAL cells CELL lines FATTY acids MICE PHOSPHORYLATION |
| Zdroj: | British Journal of Nutrition; 11/28/2023, Vol. 130 Issue 10, p1665-1677, 13p |
| Abstrakt: | The G protein-coupled receptors (GPCR) sensing nutritional signals (amino acids, fatty acids, glucose, etc.) are not fully understood. In this research, we used transcriptome sequencing to analyse differentially expressed genes (DEG) in mouse mammary gland tissues at puberty, lactation and involution stages, in which eight GPCR were selected out and verified by qRT-PCR assay. It was further identified the role of GPR110-mediating nutrients including palmitic acid (PA) and methionine (Met) to improve milk synthesis using mouse mammary epithelial cell line HC11. PA but not Met affected GPR110 expression in a dose-dependent manner. GPR110 knockdown decreased milk protein and fat synthesis and cell proliferation and blocked the stimulation of PA on mechanistic target of rapamycin (mTOR) phosphorylation and sterol-regulatory element binding protein 1c (SREBP-1c) expression. In summary, these experimental results disclose DEG related to lactation and reveal that GPR110 mediates PA to activate the mTOR and SREBP-1c pathways to promote milk protein and fat synthesis. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
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