A Rhizobium tropici DNA region carrying the amino-terminal half of a nodD gene and a nod-box-like sequence confers host-range extension.

Autor: Sousa, Carolina, Folch, Jorge Luis, Boloix, Purificación, Megías, Manuel, Nava, Noreida, Quinto, Carmen
Předmět:
Zdroj: Molecular Microbiology; Sep1993, Vol. 9 Issue 6, p1157-1168, 12p, 1 Diagram, 5 Charts, 3 Graphs
Abstrakt: Rhizobium tropici CIAT899 is a broad-host-range strain that, in addition to Phaseolus, nodulates other plant legumes such as Leucaena and Macroptilium. The narrow-host-range of Rhizobium leguminosarum biovars phaseoil (strain CE3) and trifoli (strain RS1051) can be extended to Leucaena esculents and Phaseolus vulgaris plants, respectively, by the introduction of a DNA fragment 521 bp long, which carries 128 amino acids of the amino-terminal region of a nodD gene from R tropici, as well as a putative nod-box-like sequence, divergently oriented. The 521 bp fragment, in the presence of L. esculenta or P. vulgaris root exudates, induced a R. leguminosarum bv. viciae nodA-lacZ fusion in either a CE3 or RS1051 background, respectively. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index