| Autor: |
Choi, Joseph Moon, D'Souza, Doris H. |
| Předmět: |
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| Zdroj: |
Journal of Food Science (John Wiley & Sons, Inc.); Oct2023, Vol. 88 Issue 10, p4218-4229, 12p |
| Abstrakt: |
Ongoing challenges with reproducible human norovirus cultivable assays necessitate the use of surrogates, such as feline calicivirus (FCV‐F9) and Tulane virus (TV), during inactivation studies. Chlorine alternates used as control strategies include aqueous and gaseous ozone. This study aimed at determining the inactivation of FCV‐F9 and TV by a portable ozone‐generating device. FCV‐F9 (∼8 log PFU/mL) or TV (∼6 log PFU/mL) in sterile‐low‐organic matter‐containing‐water was treated for 0–5 min, or in sterile‐water containing newborn calf serum (high‐organic matter/protein) for 0–38 min with ∼1 ppm ozone (pH 7–6). Infectivity was determined from triplicate treatments using plaque assays. FCV‐F9 titers significantly decreased by 6.07 log PFU/mL after 5 min in ozonated low‐organic‐matter‐containing‐water and was non‐detectable (≤2 log PFU/mL) after 36 min treatments in high‐organic‐matter‐containing water (p < 0.05). TV titers decreased by 4.18 log PFU/mL after 4 min in ozonated low‐organic‐matter water (non‐detectable after 4.5 min) and were non‐detectable after 22.5 min treatments of high‐organic‐matter‐containing water (p < 0.05). Overall, ∼1 ppm aqueous ozone significantly decreased FCV‐F9 by >6 log PFU/mL after 5 min, TV to non‐detectable levels (≤2 log PFU/mL) after 4.5 min and required longer treatments (>32 and >20 min, respectively) for ≥4 log reduction in high‐organic‐matter‐containing water (p < 0.05). For ozone treatment of both viruses, the linear and Weibull models were similar for low‐organic‐load water, though the Weibull model was better for the high‐organic load water. Prior filtration or organic load removal is recommended before ozonation for increased viral inactivation with decreased treatment‐time. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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