| Autor: |
小林 肇, 宮本 寛之, 渡辺 駿一, 佐藤 光三, 岩間 弘樹, 米林 英治 |
| Zdroj: |
Journal of the Japanese Association for Petroleum Technology; 2020, Vol. 85 Issue 3, p149-156, 8p |
| Abstrakt: |
Microbial source tracking (MST) is an environmental monitoring technology to trace waterborne microorganisms to the point or non-point sources. In most MST methods, DNA extracted from environmental waters are used as indicators(tracer and/or marker) of the origins of microorganisms in the samples. The usefulness of microorganisms as indicators for environmental monitoring is significantly enhanced by the recent development of DNA sequencing and data-analysis technologies. To date, MST has been implemented mainly in water management, food industry and public health sector for determining sources of fecal contamination. Yet, it also provides petroleum industry useful tools for tracking subsurface fluid flow. In this article, we introduce several examples of MST applications in oil and gas reservoirs. Particularly, through a proofo-concept trial at a natural gas field in Japan, we propose a simple procedure for data analysis applicable to various fields. DNA were extracted from produced and injected waters from the gas field. Microbial populations in each fluids were analyzed by 16S rRNA gene amplicon sequencing. Comparison of microbial compositions based on cosine similarity and k-means++ clustering revealed possible communication between wells, demonstrating that MST can provide additional insights useful to improve the operational performance. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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