| Autor: |
Faulkner, Matthew, Hoeven, Robin, Kelly, Paul P., Sun, Yaqi, Park, Helen, Liu, Lu-Ning, Toogood, Helen S., Scrutton, Nigel S. |
| Předmět: |
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| Zdroj: |
Biotechnology for Biofuels & Bioproducts; 10/11/2023, Vol. 16 Issue 1, p1-14, 14p |
| Abstrakt: |
Background: Production of relatively low value, bulk commodity chemicals and fuels by microbial species requires a step-change in approach to decrease the capital and operational costs associated with scaled fermentation. The utilisation of the robust and halophilic industrial host organisms of the genus Halomonas could dramatically decrease biomanufacturing costs owing to their ability to grow in seawater, using waste biogenic feedstocks, under non-sterile conditions. Results: We describe the isolation of Halomonas rowanensis, a novel facultative chemoautotrophic species of Halomonas from a natural brine spring. We investigated the ability of this species to produce ectoine, a compound of considerable industrial interest, under heterotrophic conditions. Fixation of radiolabelled NaH14CO3 by H. rowanensis was confirmed in mineral medium supplied with thiosulfate as an energy source. Genome sequencing suggested carbon fixation proceeds via a reductive tricarboxylic acid cycle, and not the Calvin–Bensen–Bassham cycle. The mechanism of energy generation to support chemoautotrophy is unknown owing to the absence of an annotated SOX-based thiosulfate-mediated energy conversion system. We investigated further the biotechnological potential of the isolated H. rowanensis by demonstrating production of the gaseous hydrocarbon (bio-propane), bioplastics (poly-3-hydroxybutyrate) and osmolytes (ectoine) under heterotrophic and autotrophic CO2 fixation growth conditions. Conclusions: This proof-of-concept study illustrates the value of recruiting environmental isolates as industrial hosts for chemicals biomanufacturing, where CO2 utilisation could replace, or augment, the use of biogenic feedstocks in non-sterile, industrialised bioreactors. Highlights: New halophilic isolate Halomonas rowanensis is useful for chemicals and fuel production at neutral pH. H. rowanensis is a likely facultative chemolithotroph capable of CO2 fixation in the presence of thiosulfate. Propane production occurred under both heterotrophic and autotrophic growth conditions with recombinant H. rowanensis. Potential application of H. rowanensis in the non-sterile production of chemicals and advanced synthetic fuels. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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