| Autor: |
Ghasemi, Hannah I., Bacal, Julien, Yoon, Amanda C., Tavasoli, Katherine U., Cruz, Carmen, Vu, Jonathan T., Gardner, Brooke M., Richardson, Chris D. |
| Zdroj: |
Nature Biotechnology; Oct2023, Vol. 41 Issue 10, p1398-1404, 7p |
| Abstrakt: |
We describe a strategy to boost the efficiency of gene editing via homology-directed repair (HDR) by covalently modifying the template DNA with interstrand crosslinks. Crosslinked templates (xHDRTs) increase Cas9-mediated editing efficiencies by up to fivefold in K562, HEK293T, U2OS, iPS and primary T cells. Increased editing from xHDRTs is driven by events on the template molecule and requires ataxia telangiectasia and Rad3-related (ATR) kinase and components of the Fanconi anemia pathway. The efficiency of homologous recombination is increased by crosslinking template DNA. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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