Toll‐like receptor expression profile of stem/progenitor cells from human exfoliated deciduous teeth.
Autor: | Fawzy El‐Sayed, Karim M., Rudert, Antonia, Geiken, Antje, Tölle, Johannes, Mekhemar, Mohamed, Dörfer, Christof E. |
---|---|
Předmět: |
CYTOKINES
CELL culture DECIDUOUS teeth REGENERATION (Biology) TOOTH eruption MANN Whitney U Test DENTAL pulp GENE expression profiling STEM cells DECIDUOUS dentition (Tooth development) RESEARCH funding DESCRIPTIVE statistics POLYMERASE chain reaction DATA analysis software TOLL-like receptors MESENCHYMAL stem cells |
Zdroj: | International Journal of Paediatric Dentistry; Nov2023, Vol. 33 Issue 6, p607-614, 8p |
Abstrakt: | Background: Stem/progenitor cells from human exfoliated deciduous teeth (SHED) show remarkable pluripotent, regenerative, and immunological capacities. During in vivo regenerative processes, there could be the presence of SHED in the surrounding inflammatory microenvironment, through toll‐like receptors (TLRs). Aim: The aim of this paper was to present a characteristic TLR expression profile on SHED for the first time. Design: Cells were harvested from extracted primary teeth (n = 10), anti‐STRO‐1 immunomagnetically sorted and cultivated, through colony‐forming units (CFUs). SHED were examined for mesenchymal stem/progenitor cell traits, including the expression of clusters of differentiation (CDs) 14, 34, 45, 73, 90, 105, and 146, and their multilineage differentiation aptitude. TLRs 1–10 expression was investigated for SHED in uninflamed and inflamed (25 ng/mL IL‐1β, 103 U/mL IFN‐γ, 50 ng/mL TNF‐α, and 3 × 103 U/mL IFN‐α; SHED‐i) microenvironmental conditions. Results: SHED were negative for CDs 14, 34, and 45, but were positive for CDs 73, 90, 105, and 146, and demonstrated characteristic multilineage differentiation. In an uninflamed microenvironment, SHED expressed TLRs 1, 2, 3, 4, 6, 8, 9, and 10. The inflammatory microenvironment downregulated TLR7 significantly on gene level and upregulated TLR8 on gene and protein levels (p <.05; Wilcoxon signed‐rank test). Conclusion: There appears to be a unique TLR expression profile on SHED, which could modulate their immunological and regenerative abilities in oral tissue engineering approaches. [ABSTRACT FROM AUTHOR] |
Databáze: | Complementary Index |
Externí odkaz: |
Pro tento záznam nejsou dostupné žádné jednotky.