| Autor: |
Fedorova, N. D., Sumbatyan, D. A., Sokolov, A. V., Filatov, M. V., Trashkov, A. P., Varfolomeeva, E. Yu. |
| Zdroj: |
Biophysics; Jun2023, Vol. 68 Issue 3, p416-421, 6p |
| Abstrakt: |
Neutrophils are the leading cells of the innate immune system and the main population of leukocytes responsible for the primary reaction of the body to various infectious particles. The latter are destroyed by neutrophils due to the processes of phagocytosis and a cascade of reactions, including a respiratory burst reaction. As a result of the respiratory burst reaction of neutrophils they produce reactive oxygen and halogen species, powerful cytotoxic agents that destroy particles in the phagolysosome. All of these processes require regulation, because overactivation of neutrophils can lead to the damage of the tissues surrounding the focus of inflammation mediated by the reactive forms of oxygen. Proteins of the acute phase of inflammation claim for the role of inflammatory regulators. Previously, we have shown the participation of ceruloplasmin in inhibiting the respiratory burst reaction of neutrophils in blood samples. Fibrinogen, on the contrary, increased the intensity of the respiratory burst reaction. Not for all proteins of the acute phase of inflammation and especially their combinations, the effect on the functions of neutrophils has been studied in detail. In this work the effect of a number of proteins of the acute phase of inflammation (C-reactive protein, serum amyloid A, alpha-1-acid glycoprotein and fibrinogen) on the ability of peripheral blood neutrophils to the respiratory burst reaction was studied for the first time using flow cytometry with the registration of the production of reactive oxygen species in cells of peripheral blood samples. Significant changes in the ability of neutrophils to produce reactive oxygen species were found for a number of combinations of the studied proteins of the acute phase of inflammation. The study of interactions of ceruloplasmin and fibrinogen with peripheral blood neutrophils revealed their membrane localization. It seems promising to identify receptors for proteins of the acute phase of inflammation on the neutrophil membrane. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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