| Autor: |
Petrova, P. A., Zamalutdinova, S. V., Vnukova, A. A., Alekseeva, D. A., Bagrov, D. V. |
| Zdroj: |
Biophysics; Jun2023, Vol. 68 Issue 3, p341-346, 6p |
| Abstrakt: |
Plasma treatment of a surface can render it hydrophilic and improve its adsorption properties. Here, it is shown that the glow discharge treatment of nitrocellulose membranes improved the adsorption of antibodies by 15–17 times and increased the sensitivity of immunoassay (dot blotting) performed on the membranes by about an order of magnitude. This feature was demonstrated for the interleukin-1 beta immunoassay with chemiluminescent detection. Glow discharge treatment of nitrocellulose membranes was not accompanied by changes in their structure or chemical composition; it was shown using scanning electron microscopy and IR spectroscopy, respectively. The obtained results can be used to optimize the existing laboratory procedures, especially dot blotting. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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