| Autor: |
Septiyanti, Melati, Agustian, Egi, Meliana, Yenny, Ghozali, Muhammad, Triwulandari, Evi, Sampora, Yulianti, Devy, Yenni Apriliani, Sondari, Dewi, Septevani, Athanasia Amanda |
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| Zdroj: |
AIP Conference Proceedings; 2023, Vol. 2902 Issue 1, p1-12, 12p |
| Abstrakt: |
Bacterial growth is one of the important factors in food safety. Fresh food such as fruits, vegetables, and meat are vulnerable to pathogen microbes such as Escherichia coli, Staphylococcus aureus, and Bacillus subtillis. To keep the good quality of food, the preservative is commonly used as a food additive. One of the natural antibacterial substances comes from citronella oil and its fraction. However, direct usage of citronella oil only lasts for a short time because of diffusion or interaction with the food product. To overcome this limitation, citronella oil can be incorporated into polymer packaging such as Polylactic Acid (PLA). This research aims to investigate the antibacterial activity of citronella oil and its fractions namely rhodinol, citronellal, and limonene on PLA as food packaging and understand its ability to inhibit bacterial growth on fresh and processed meat, as well as analyze its biodegradability. Antibacterial activity was evaluated through the zone inhibition method against S. aureus, B. subtillis, and E. coli. PLA/Citronella oil film was made through solvent casting using chloroform, and the biodegradability test was conducted qualitatively using Aspergillus niger according to ASTM G21-96. The bacterial growth on fresh and processed meat was tested using the total plate count (TPC) method. The rhodinol and limonene fractions in chloroform showed the highest antibacterial activity at a concentration of 5-10%. PLA films with 10% rhodinol and limonene respectively inhibited bacterial growth in both fresh and processed meat, with TPC<10000 CFU/cm2 for fresh meat and TPC<2500 CFU/cm2 for processed meat. The films also showed biodegradability in a 30-day test period, with the limonene film showing higher biodegradability than rhodinol. The PLA film with the addition of citronella oil especially rhodinol and limonene showed favorable antibacterial activity and was able to preserve the meat sample against microbial growth in ambient temperature. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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