Preparation of a Single-Cell Suspension from Tumor Biopsy Samples for Single-Cell RNA Sequencing.

Autor: Frolova, A. A., Gerashchenko, T. S., Patysheva, M. R., Fedorov, A. A., Tsyganov, M. M., Bokova, U. A., Bragina, O. D., Vostrikova, M. A., Garbukov, E. Yu., Cherdyntseva, N. V.
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Zdroj: Bulletin of Experimental Biology & Medicine; Aug2023, Vol. 175 Issue 4, p519-523, 5p
Abstrakt: An essential requirement for single-cell RNA sequencing in cancer is the preparation of high-quality single-cell suspensions from the tumor tissue. In this work, various methods of dissociation of tumor biopsy specimens were analyzed and developed to obtain a cell suspension with at least 80% viability. It was found that the optimal conditions for sample preparation are mechanical dissociation followed by incubation with a collagenase/hyaluronidase mixture with addition of DNAase I for 60 min. Thus, we optimize the approach for preparing single-cell suspensions from the tumor biopsy tissue for single-cell RNA sequencing. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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