| Abstrakt: |
It is impossible to reliably evaluate the ecotoxicity of small subtoxic doses of dioxins without duly taking into account how the factors of real exposure conditions affect their properties. The best approach in this regard may be biomonitoring of the initial manifestations of the toxic effects. In the present work, the authors studied bank voles (Clethrionomys glareolus) from the natural population living close to a dumpsite, and more specifically the summer-born adults and overwintered functional groups of animals that differed by the dioxin burden. Voles from the vivarium-kept line served as a control. The initial toxic effects were characterized by the expression levels of the following genetic markers: ahr, cyp1a2, keap1, dnmt1, dnmt3a, dnmt3b, LINE-1 and B1-SINE. The expression levels of the ahr, keap1, dnmt3a, and dnmt3b genes were statistically higher in the summer-born adult group than in the control voles. The expression of the cyp1a2 and keap1 genes was increased in the overwintered group compared to the control, while no differences were detected for dnmt1, LINE-1, and SINE B1. The increase in the expression of the marker genes observed in the dioxin-exposed voles is in good correspondence with the mechanisms of formation and development of toxicological effects under the conditions of low-dose toxin action on several generations in the population. Our findings will contribute to the development of an evaluation technique that could be used in biomonitoring of the initial ecotoxicity effects in the dioxin-contaminated environment. [ABSTRACT FROM AUTHOR] |
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