| Autor: |
Parker, Matthew F L, López-Álvarez, Marina, Alanizi, Aryn A, Luu, Justin M, Polvoy, Ilona, Sorlin, Alexandre M, Qin, Hecong, Lee, Sanghee, Rabbitt, Sarah J, Pichardo-González, Priamo A, Ordonez, Alvaro A, Blecha, Joseph, Rosenberg, Oren S, Flavell, Robert R, Engel, Joanne, Jain, Sanjay K, Ohliger, Michael A, Wilson, David M |
| Předmět: |
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| Zdroj: |
Journal of Infectious Diseases; 2023 Supplement, pS281-S290, 10p |
| Abstrakt: |
Background Vertebral discitis-osteomyelitis (VDO) is a devastating infection of the spine that is challenging to distinguish from noninfectious mimics using computed tomography and magnetic resonance imaging. We and others have developed novel metabolism-targeted positron emission tomography (PET) radiotracers for detecting living Staphylococcus aureus and other bacteria in vivo, but their head-to-head performance in a well-validated VDO animal model has not been reported. Methods We compared the performance of several PET radiotracers in a rat model of VDO. [11C]PABA and [18F]FDS were assessed for their ability to distinguish S aureus , the most common non-tuberculous pathogen VDO, from Escherichia coli. Results In the rat S aureus VDO model, [11C]PABA could detect as few as 103 bacteria and exhibited the highest signal-to-background ratio, with a 20-fold increased signal in VDO compared to uninfected tissues. In a proof-of-concept experiment, detection of bacterial infection and discrimination between S aureus and E coli was possible using a combination of [11C]PABA and [18F]FDS. Conclusions Our work reveals that several bacteria-targeted PET radiotracers had sufficient signal to background in a rat model of S aureus VDO to be potentially clinically useful. [11C]PABA was the most promising tracer investigated and warrants further investigation in human VDO. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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