| Autor: |
DMITRIC, MARKO, VIDANOVIC, DEJAN, MATOVIC, KAZIMIR, TESOVIC, BOJANA, SEKLER, MILANKO, VICIC, IVAN, KARABASIL, NEDJELJKO |
| Předmět: |
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| Zdroj: |
Czech Journal of Food Sciences; 2023, Vol. 41 Issue 4, p287-294, 8p |
| Abstrakt: |
In this study, the primers and the probe were designed, and a completely new real-time polymerase chain reaction (PCR) protocol for detecting the invA gene of Salmonella in food was optimised and validated in-house. The inclusivity test used 76 different Salmonella isolates with no false-negative results. The exclusivity was tested using 45 non-Salmonella microorganisms with no false-positive results. The method was also successfully applied while examining five different artificially contaminated food categories. The results were compared to the standard method (ISO 6579-1) and two previously validated real-time PCR methods. The developed assay is sensitive and specific for rapidly detecting Salmonella in food. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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