| Abstrakt: |
Candida is one of the most common causes of fungal diseases, these diseases range from superficial infections to systemic infections. The study included the cultivation of three types of Candida on a broth nutrient medium To determine the biofilm concentration resulting from the growth of these three species under vibration incubation conditions as well as under static incubation conditions using the microtiter dish technique. The result showed the biofilm mean concentrations are 0.096, 0.090, and 0.081 UI/ml of C. albicans, Candida dubliniensis, and candida spherical respectively in the shaking state while the biofilm means concentrations are 1.012, 1.231, and 1.183 UI/ml of C. albicans, Candida dubliniensis, and candida spherica respectively in a stable state compared to control (only nutrient broth), the study also found that Candida albicans is more than the rest of the other species in the biofilm formation. Finally, we can conclude that the development of Candida on culture media in stable conditions leads to an increase in the formation of biofilm and we found that Candida albicans is the most susceptible to biofilm formation, with its virulence factors, adaptability, and transformation from one phase to another, which is dimorphic. This study is the first GC/MS analysis of biofilms of Candida albicans, Candida dubliniensis, and Candida spherica. Many compounds have been identified in the GC/MS Instrument Library database. [ABSTRACT FROM AUTHOR] |
