| Autor: |
Bordoloi, Kuntala Sarma, Baruah, Pooja Moni, Tanti, Bhaben, Gill, Sarvajeet Singh, Agarwala, Niraj |
| Předmět: |
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| Zdroj: |
Journal of Plant Growth Regulation; Oct2023, Vol. 42 Issue 10, p6523-6548, 26p |
| Abstrakt: |
Helopeltis theivora or the tea mosquito bug (TMB) is reportedly one of the most devastating pests of tea plant (Camellia sinensis) causing threat to the beverage crop. Long non-coding RNAs (lncRNAs) constitute a group of endogenous RNAs that play gene regulatory roles in eukaryotes. In the present study, 9502 lncRNAs were identified from healthy and TMB-infested C. sinensis tissues using high-throughput strand-specific RNA sequencing, out of which 80 lncRNAs got differentially expressed in response to TMB infestation. Determination of genes that could act as potential targets of lncRNAs revealed that the identified lncRNAs could possibly target as many as 5804 genes. Differential gene expression (DGE) analysis led to the identification of 3665 differentially expressed genes (DEGs), of which, the expression of 1767 genes got upregulated and 1898 genes got downregulated during tea plant's response to TMB. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of DEGs and lncRNA-target genes have shown that TMB infestation might have triggered transcriptomic reprogramming leading to altered primary and secondary metabolism in C. sinensis. LncRNAs can act as competing endogenous RNAs (ceRNAs) to bind with common microRNA (miRNA) response elements (MREs) involving a competition between mRNAs and lncRNAs. We report 11 lncRNAs competing with 14 mRNAs to bind with 28 miRNAs forming the ceRNA network. The expression of 6 DEGs and 5 differentially expressed lncRNAs (DELs) has been validated by qRT-PCR. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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