| Autor: |
Minina, Yu. M., Soroka, A. B., Karamysheva, T. V., Serdyukova, N. A., Serov, O. L. |
| Zdroj: |
Cell & Tissue Biology; Oct2023, Vol. 17 Issue 5, p557-564, 8p |
| Abstrakt: |
In modern molecular and cellular biology, CRISPR/Cas9 technology has been widely used for targeted modification of human and animal genomes. In this work, using molecular-cytogenetics methods, we have analyzed the karyotype in 18 mouse fibroblast cell lines (MFs), whose genome had a Cntn6 gene edited with CRISPR/Cas9. The modifications of Cntn6 gene were 2374-kb duplications, 1137-kb deletions, and inversions of similar size. In addition, cytogenetic analysis was performed for five control lines of mouse embryonic fibroblasts (MEFs) carrying an intact Cntn6 gene allele. The study showed the presence of a high level of polyploidy for MF lines that were heterozygous for Cntn6 gene inversions and homozygous and heterozygous for Cntn6 gene duplications (20–46%), as well as monosomy (1–9%) and trisomy (1–8%) for chromosome 6. It is important to note that no trisomy was detected in MEF lines carrying a deletion and duplication of Cntn6 gene in the compound, and the proportion of polyploid cells was minimal (1.5–5.7%). Thus, the data obtained indicate the destabilization of the karyotype of cell lines that underwent genome editing by CRISPR/Cas9. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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