| Abstrakt: |
Kelus (Kelussia odoratissima Mozaff.) is one of the medicinal, spicy, aromatic and endemic herbs that grows in restricted regions of Iran. Due to the importance of its pharmaceutical compounds in traditional medicine, the excessive harvesting has led to a nearly risk of extinction. To protect kelus from extinction, plant tissue culture can be used to produce useful compounds and secondary metabolites. The hairy root culture is an efficient system for the production of medicinal compounds and valuable secondary metabolites in plants. For this purpose, two pathogenic strains of Agrobacterium rhizogenes (A4 and ATCC15834), two types of explants (cotyledon and hypocotyl), three levels of acetosringon (a1= 0 μM, a2=50 μM, and a3= 100 μM), and three levels of glucose (b1=0, b2=0.5%, b3=1%) were applied using a factorial experiment based on a completely randomized design with three replications. The results of this study showed that both Agrobacterium strains were able to induce hairy roots production in kelus. Also, ATCC15834 strain in MS medium with 100 μM of acetosringon in the presence of 0.5% glucose had the highest effect in producing hairy roots from cotyledon explants. The lowest amount of hairy roots was observed for A4 strain in MS medium with no acetosringon and glucose added. PCR analysis was performed with rolC specific primers to ensure hairy root induction by A. rizhogenes. The results of this part of the study, for the first time in Iran, showed that it was possible to induce hairy roots in kelus explants, paving the way to propagate kelus in vitro. [ABSTRACT FROM AUTHOR] |
