| Autor: |
Bini, Markose, Rajesh, Bhargavan, Babu, Thekkekara Devassy |
| Předmět: |
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| Zdroj: |
Journal of Basic & Clinical Physiology & Pharmacology; Sep2023, Vol. 34 Issue 5, p617-623, 7p |
| Abstrakt: |
Calcium carbide (CaC2) and ethylene glycol (EG) are the two commonly used fruit ripening agents. The toxic effects of these chemicals on internal organs were reported in experimental animals. Even though the adverse effects of these compounds have been investigated for many years, there are no sufficient data available with regard to genotoxic effects. The present study evaluates the genotoxic effect of chronic exposures of CaC2 and EG in Wistar albino rats. CaC2 and EG were administered to the rats orally for 180 days. Chromosomal aberrations and micronuclei formation were analysed in bone marrow and peripheral blood cells. Comet assay was performed to analyse the DNA strand break. The toxic effects of the chemicals were analysed by MTT assay with normal human intestinal epithelial (IEC-6) cells. Upon chronic exposure, CaC2 and EG caused chromosomal aberrations, micronuclei formation and DNA strand breaks extensively in bone marrow and peripheral blood cells. In MTT assay, the chemicals were found to be toxic to IEC-6 cells with IC50 values at 160 and 200 μg/mL for CaC2 and EG, respectively. The results show that these chemicals have a potential to cause genomic level of toxicity which may lead to carcinogenic event at a chronic level exposure. The study warns to reinforce the administrative measures against the use of CaC2 and EG for fruit ripening process. – Industrial grade CaC2 and EG show genotoxicity at the chronic exposure in animals at lower doses. – In vitro cytotoxicity by CaC2 and EG towards IEC-6 cell line exhibited dose dependent decrease of cell proliferation. – Chromosomal aberrations, micronuclei formation, DNA breakage (comet assay) suggest potentially pre-mutagenic lesions by the toxicity. – These chemicals have the potential for genotoxic and carcinogenic effect. – The study warns the health risks of consumption of fruits ripened with calcium carbide and ethylene glycol. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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