| Abstrakt: |
A new facultative methylotroph, strain 6x-1T, was isolated from the phyllosphere of oak (Quercus pubescens Willd.) on the medium with methanol. Cells were aerobic, gram-negative, not-spore-forming, non-motile short ovoids rods reproducing by binary fission. Growth optimum was at 25‒29°C and pH 7.0‒7.5; growth was inhibited by 1.5% NaCl. Predominant fatty acids were C18:1ω7c and C19:0cyclo. Predominant phospholipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, and diphosphatidylglycerol. The major ubiquinone was Q10. The 16S rRNA gene sequence of strain 6x-1T exhibited the highest similarity to those of members of the genus Ancylobacter (97.0‒97.4%). Genome analysis of strain 6x-1T and most closely related Ancylobacter strains revealed that the values of ANI (80.7‒83.5%), dDDH (22.4‒23.3%), AAI (72.0‒78.0%), and POCP (62.0‒69.0%) were below the recommended threshold values for prokaryotic species. Genome size of strain 6x-1T was 4.29 Mb, and G + C content was 67.3%. According to the results of phylogenetic, phylogenomic, phenotypic, and chemotaxonomic analysis, strain 6x-1T (=(VKM В-3256T = ССUG 72401T = КСТС 92567T)) represents a new species of genus Ancylobacter, for which the name Ancylobacter crimeensis sp. nov. is proposed. [ABSTRACT FROM AUTHOR] |
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