Isolation, identification and optimization of potential phenol degrading bacterial strain P7 using Gen III microlog.

Autor: Bandi, Sai Spandana, Mallisetty, Rishi, Veluru, Sridevi, Hamzah, Husam Talib, Poiba, Venkata Rao, Srikanth, Rachakonda
Předmět:
Zdroj: AIP Conference Proceedings; 2023, Vol. 2764 Issue 1, p1-9, 9p
Abstrakt: Phenol and phenolic compounds are one of the top pollutants in environment due to their presence in various industrial effluents and their broad applications in various other fields. The results of this current study proved that isolated strain P7 is capable of degrading high capacity phenol. Optimal conditions obtained for the degradation of phenol from this strain created a solid foundation for further use of this microorganism in the treatment of phenol effluents. Studies were conducted out using Mineral Salt Agar Medium for the growth of this strain and degradation of phenol using phenol as the sole carbon and energy source for the isolated bacterial strain. The strains obtained from the phenol contaminated soil were designated as P1, P2, P3, P4, P5, P6, P7 & P8. P7 strain among these was found to be highly effective in removing phenol. Gen III biolog was used to identify the name of this P7 strain species, which is isolated from soil sample. Gen III Biolog, a phenotypic microarray identified the strain P7 as Pseudomonas Putida. Optimization studies were carried out to find out the optimum pH, temperature, inoculum volume, contact time and initial phenol concentration for the Pseudomonas Putida species. The optimum conditions obtained for maximum phenol removal were found to be pH of 7.5, temperature of 32OC, Contact time of 72hrs, phenol concentration of 200 mg/l and inoculum volume of 4v/v. The maximum biodegradation rate achieved was 93.29% from this isolated strain which indicates its potential use in the bioremediation of wastewater effluent. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index