| Abstrakt: |
The study aimed to produce mycodiesel using cheap and available sources, based on four fungal Oleaginous species that were represented by: Aspergillus fumigatus strain ab, Aspergillus terreus strain KAIN1, Cladosporium ramotenellum strain ZST3 and Lichtheimia corymbifera strain K16. Fungi were cultured in three different fermentation media which included cheese whey medium (CWM), waste molasses dates medium (WMDM), and nitrogen limiting medium (NLM), for biomass production. AS well as, lipids were extracted from the dried produced biomass, by using methanol with chloroform as extraction solvents. While, FAMEs from extracted total lipids were detected by using Gas Chromatography-Mass Spectrometry (GC/MS), after transesterification reactions. On the other hand, some important characteristics of mycodiesel produced quality such as cetane number (CN), carbon residue (CR), and sulfur content (SC) were measured. In the present study, the biomass production and lipids accumulation of Oleaginous fungi were showed a significant difference p< 0.05 in the three fermentative media. Where Cladosporium ramotenellum and Aspergillus terreus gave the highest dry weight by (16.08 g L−1) and (15.48 g L−1) respectively in NLM, while the lowest dry weight was due to, Lichtheimia corymbifera by (12.8 g L−1) in the same media. As well as, Oleaginous fungi were good grown in natural fermentation media, by CWM, Lichtheimia corymbifera were produced (40 g L−1) dry weight. While Aspergillus fumigatus and Aspergillus terreus were produced (6.4 and 4.58 g L−1) dry weight respectively in WMDM. Furthermore, in NLM, Aspergillus fumigatus was accumulated (26%) of lipids, followed by Aspergillus terreus (24.9%),while, in CWM Aspergillus terreus was accumulated (38%), as well as Cladosporium ramotenellum and Aspergillus fumigatus were accumulated (24 %) of lipids in WMDM. The percentage of produced FAMEs were showed significant differences p< 0.05 between fungal isolates and the type of fatty acids. The results of fatty acids were included Myristic acid, Palmitic acid, Stearic acid, Oleic acid, Linoleic acid, and Linolenic acid. Moreover, the Oleaginous fungi gave varying values of CN. In addition, the mycodiesel produced were showed very low values for CR and SC after the combustion process. [ABSTRACT FROM AUTHOR] |
