| Abstrakt: |
The polypeptides from fruiting bodies of Volvariella volvacea were extracted by alkaline protease enzymatic hydrolysis, and purified by dialysis method to yield three different components (3 to 10, 1 to 3, and less than 1 kDa). The antioxidant activities in vitro of the three components were compared. The structure of purified polypeptide was characterized by LC-MS/MS, ultraviolet absorption spectroscopy, Fourier transform infrared spectroscopy, and amino acid determination methods. The result showed that the antioxidant activity of 1 to 3 kDa was strongest. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical clearance rate was 99.81%, the ferric ion reducing ability was 1.47, and the clearance rates of 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+ ) radical and hydroxyl radical were 99.15% and 99.34%, respectively. The total protein content of the antioxidant peptide of V. volvacea at this molecular weight was 71.12%, ash content was 12.75%, moisture content was 8.44% and the others were 7.69%. The results of structural identification showed that the polypeptides from fruiting bodies of V. volvacea were high quality protein materials. [ABSTRACT FROM AUTHOR] |
